稀有鮈鲫对草鱼出血病病毒敏感性的初步研究

PRELIMINARY STUDY ON THE SUSCEPTIBILITY OF GOBIOCYPRIS RARUS TO HEMORRHAGIC VIRUS OF GRASS CARP(GCHV)

本文报道稀有鲫对草鱼出血病病毒（ＧＣＨＶ）的敏感性。ＧＣＨＶ是草鱼出血病的病原，用ＧＣＨＶ人工感好１－６月龄的稀有鲫，在水温２２－３２℃时能导致稀有鲫出现出血病症状。在水温２８℃时，病鱼在ｌｄ内死亡，潜伏期为５ｄ，发病高峰期在感染后第６－８ｄ。ＧＣＨＶ能在稀有鲫体内传代，并诱导８０％以上的稀行鲫患病死亡。将人工感染ＧＣＨＶ的稀有鲫病鱼组织超薄切片，电镜观察，发现在肠道、脾脏、肾脏等组织中存在大小与形态和ＧＣＨＶ相似的病毒颗粒。从稀有鲫出血病病鱼组织中纯化病毒，免疫电镜观察，发现病毒颗粒能被ＧＣＨＶ的特异性抗体聚集成团。由上可知，稀有鲫出血病是由ＧＣＨＶ感染所致，稀有鲫对ＧＣＨＶ是敏感的，可以作为草鱼抗出血病育种的模型。

This is the report on the susceptibili ty of Gobiocypris rarus to Hemorrhagic Virus ofGrass Carp(GCHV). The hemorrhage of GobiocJlpris rarus is induced by the GCHV in theage from 1-6months when the water temperature is 22℃-32℃. At the water temperatureof 28℃, the diseased fish die in one day, whose latent period is 5 days after infection, The pe-riod of high mortality of the diseased fish comes in 6-8days after the infection, GCHV cantransmit in Gobiocypris rarus, and more than 80% of the fish die from the infection ofGCHV. The electron microscopic examination on the uItrathin sections reveals that there arevirus particles, which resemble GCHV in size and shape, in such organs as the intestinaltract, the spleen, and the kidney etc. of the artifficially infected hemorrhagic fish. Theimmuno-electron microscopic observation showed that the purified virus particles isolatedfrom the hemorrhagic Gobiocypris raru,,can be aggregated by the specific GCHVantibody. The resuIts prove that the hemorrhage of Gobiocypris rarus is caused by GCHV,and that Gobiocypris rarus is susceptible to GCHV, Furthermore, GobiocyPris rarus mayserve as a model of tlie Virus-resistance breeding of Grass Carp with biotechnique methods.