3种淡水育珠蚌碱性磷酸酶（ALP）比较酶学的初步研究

STUDY ON THE COMPARISON OF ALKALINE PHOSPHATASE(ALP)FRO TMREE FRESHWATER PEARL MUSSELS

分别从三角帆蚌、褶纹冠蚌、背角无齿蚌的外套膜中部分提取了一种碱性磷酸酶（ＡＬＰ），并对这３种来源的ＡＬＰ进行了比活力和动力学性质的比较研究。经过相同提纯步骤后，外套膜ＡＬＰ比活顺序是褶纹冠蚌＞背角无齿蚌＞三角帆蚌；三角帆蚌ＡＬＰ作用于底物磷酸苯二钠的最适ｐＨ值为９．４０，最适温度为３７℃，米氏常数Ｋｍ为０．５３ｍｍｏｌ／Ｌ；褶纹冠蚌ＡＬＰ的最适ｐＨ值为９．１２，最适温度为４０℃，米氏常数Ｋｍ为１．７２ｍｍｏｌ／Ｌ；背角无齿蚌ＡＬＰ的最适ｐＨ值为９．５０，最适温度为４０℃，米氏常数Ｋｍ为０．５７ｍｍｏｌ／Ｌ。３种蚌的ＡＬＰ均为Ｍｇ￣（２＋）、Ｃａ￣（２＋）、Ｂａ￣（２＋）所激活，为Ｃｕ￣（２＋）、Ｚｎ￣（２＋）、ＫＨ＿２ＰＯ＿４、ＭＥ（疏基乙醇）、ＥＤＴＡ－Ｎａ＿２所抑制。

An alkaline phosphatase was partly purified from the pallium of Hyriopsis cuminigii(Lea),Cristaria plicata (Leach),Anodonta woodiana (Lea)respectivel. The ALPs of different sources were compared under the same conditions.The results are as follows:After the same purification steps,the order of the specific activity is:Cristarta plicata>Anodonta woodiana>Hyriopsis cumingii.The optimal pH and temperature and Km of Hyriopsis cumingil’s ALP for the hydrolysis ofdisodium phenol phosphate are:pH9.40,37℃and 0.5 3mmol/L; the data of Cristaria plicata’sALP are:pH9. 12,40℃and 1.7 1mmol/L;the data of Anodonta woodiana’s ALP are pH9. 50,40℃ and 0.57mmol/L.The ALPs of these mussels are all activated by Mg￣(2+), Ca￣(2+) and Ba￣(2+) but inhibited by Cu￣(2+),Zn￣(2+),KH_2PO_4,ME(2-mercaptoethnol)and EDTA-Na_2.