PAR比色法直接测定血清铁

DETERMINATION OF SERUM IRON BY DIRECT SPECTROPHOTOMETRY

４－（２－吡啶偶氮）间苯二酚（ＰＡＲ）与Ｆｅ２＋生成红色水溶性化合物。最大吸收峰为５００ｎｍ和７１５ｎｍ，用７１５ｎｍ作工作波长可提高选择性。线性范围０～７１．６ｕｍｏｌ／Ｌ，表现摩尔吸光系数为２．４８×１０４Ｌｍｏｌ－１ｃｍ－１ψ＝０．９９９６，测定４８例健康成人血清铁Ｘ１９．９３ｕｍｏｌ／Ｌ，正常参考值范围为１１．８～２８．０μｍｏｌ／Ｌ，平均回收率为１００．２％，批内ＣＶ为２．４％，批间ＣＶ为４．２％，与微机示波极谱法比较结果无差别（Ｐ＞０．０５）。该法灵敏、准确、选择性高，血清用血量少，不必除去蛋白，操作简单快速。

pyridyazo) resorcinol and iron will produce a red soluble compound.The maximum absorption wavelength was 500 nm and 715nm. But using the latter will promote determinational selectivity.The linear range was from 0.0 to 71.6μmol/L. Absortion coefficient was 2. 48×104 L. mol-1·cm-1,and correlative coefficient was 0. 9996.According to determinat 48 case of healthy adults,the results shows that the mean was 19.93 μmol/L. thenormal reference value was 11.8 to 28.0 umol/L,average of recovery rate was 100.2%,coefficient of variation in same sample was 2.4% and coefficient of variation in different sample was 4.2%,compared with computered osillograph polarography. The results showed no statistical difference (P>0.05).The method was a sensible. precise,distinctive one, need a small amount of sample, not necessary to get rid of protein, and the operation was simple andquick.