金黄色葡萄球菌的分离鉴定及药敏试验

SEPARATION AND IDENTIFICATION OF STAPHYLOCOCCUS AUREUS AND ITS DRUG-SENSITIVITY TEST

从临床标本中共分离出金黄色葡萄菌７６株，其中耐甲氧西林金黄色葡萄球菌（ＭＲＳＡ）３２株，占４２％；对甲氧西林敏感的金黄色葡萄菌（ＭＳＳＡ）４４株，占５８％，同时用９种常用药物对金黄色葡萄球菌进行了药敏试验。发现万古霉素、氟哌酸、丁胺卡那，卡那霉素对ＭＳＳＡ敏感率分别为：１００％、１００％、７３％、５５％；万古霉素、氟哌酸对ＭＲＳＡ的敏感率分别为：８８％、５０％。对９种抗生素的耐药率ＭＲＳＡ显然高于ＭＳＳＡ（Ｐ＜０．０５）。产β─内酰胺酶ＭＲＳＡ为６９％高于ＭＳＳＡ的４５％，总产酶率为６１％。

Seventy-six strains of staphylococci Aureus were separated from clinical samples,of which 32 strains(42%) were methicilin-resistant (MRSA) while 44 strains(58%) being methicilin sensitive(MSSA).Drug-sensitivity tests were done on 9 variety of drugs indicating that the sensitivity rates of vancomycin, Norfloxacin,amikacin and kanamycin were 100%,100%,73%and 55%to MSSA respectively while that of vancomycin and Norfloxcin being 88%and 50%to MRSA.The drug-resistant rate of MRSA is significantly higher thanthat of MSSA (P<0.05).The production of β-endoamidase from MRSA was 69% which was higher than that of MSSA,The total ezyme yielding rate was 61%.