自制氰化高铁血红蛋白参考标准液的质量评价及初步应用

QUALITY EVALUATION AND PRIMARY USES OF THE HICN REFERENCE STANDARD MADE IN OUR LABORATORY

对自制氰化高铁血红蛋白参考标准液进行了质量评价及初步应用。结果表明：此参考标准液的吸收曲线特征、Ａ５４０／Ａ５０４比例，波长７５０ｍｍ处吸光度、包装及无菌试验均达到ＩＣＳＨ标准；与上海医化所同类产品比较线性和准确度符合性好；４－８℃保存３８月后其浓度最大波动为３．７％，平均２．５％。此标准液应用于５８１光电比色计及７２１、７２２分光光度计上作标准曲线以测定血红蛋白浓度，作为电子血细胞计数仪和血红蛋白仪测定血红蛋白时的校正液结果满意。

Quality evaluation studies and primary clinical uses of the HICN reference standard made in our laboratory are reported in this paper. Results prove that it is satisfactory in the characterostocs of absorbance curve,A540/A504 ratio, absorbance at 750nm, packing and bacterial culture test,all these parameters reaching the standards of ICSH,Comparing the linearity and accuracy with those of the products made in Shanghai Medical Laboratory Institute, the results of our products are in good agreement with them. Having stored for 38 months at 4～8℃ the HICN concentration only decreased 3.7%at most.2.5%in average. The reference standards have been used on 581,721,722 electrophotometers to make standard curve and on electron blood cell counter and hemoglobinometer as calibration solution for determination of Hb,results are satisfactory as well.