聚合酶链反应检测血清中HBV DNA的临床意义

Detection of Serum Hepatitis B Virus DNA with the Polymerase Chain Reaction

应用聚合酶链反应(Ploymerase Chain Reaction,PCR)检测临床中感染乙型肝炎病毒(HBV)者的带毒状况。将乙型肝炎病毒血清标志物(HBVM)与PCR检测结果进行比较,探索血清中HBV DNA的临床意义。结果表明PCR方法在HBV DNA的检出优于HBVM(P<0.01)。阐明即使血清中有保护性抗体出现也可能有低水平的HBV存在。有条件的医院应同时用HBVM及PCR进行检测,可以更有效地阻断HBV传播途径,确保血源质量,减少输血后肝炎的发生。

Serum HBV DNA was tested in 218 cases with polymerase chain reaction (PCR) and ELISA. The results analysed showed that there were 29 cases PCR positive which accounted for 59.18% in 49 cases with HBsAg positive, 15 caces PCR positive in 74 cases with Anti-HBs positive. According to the results of comparing HBVM with PCR, the HBsAg negative in some Patients indicated that HBV were in a state of replication and expression. The writer suggested that both HBVM and PCR be tested at the same time.