HPLC法测定牛黄消炎片中脂蟾毒配基、华蟾酥毒基的含量

HPLC Determination of Redbufogenin and Cinobufagin in Niuhuang Xiaoyan Tablets

目的:建立高效液相色谱法测定牛黄消炎片中脂蟾毒配基、华蟾酥毒基的含量。方法:色谱柱为Kromasil C_(18)柱(250mm×4.6 mm,5 μm),以乙腈-0.5%磷酸二氢钾溶液(50:50)为流动相,流速为1.0 mL·min^(-1),检测波长为296 nm,进样量为20μL。结果:脂蟾毒配基的线性范围为0.18—2.80μg(r=0.9999),华蟾酥毒基的线性范围为O.19—2.90μg(r=0.9999),平均回收率分别为98.4%和100.6%。结论:本方法简便、稳定、可靠、重现性好,可用于控制成品质量。

Objective:To establish an HPLC method for the determination of recibufogenin and cinobufagin in Niuhuang Xiaoyan tablets.Methods:This methods used Kromasil C_(18)column(250 mm×4.6mm,5 μm)with acetonitrile-0.5% potassium dihydrogen phosphate solution of water (50:50) as the mobile phase, 1.0 mL·min^(-1)as flow rate,296 nm as detection wavelenth,20μL as the injection volume.Results:The method offered good linearity 0.18 -2.80μg for recibufogenin(r=0.9999),and 0.19-2.90μg for cinobufagin(r=0.9999).The average recovery rates were 98.4% and 100.6% respectively.Conclusion:The method is simple, accurate, reproducible and can be used for quality control of this preparation.