RP-HPLC测定地稔药材中没食子酸的含量

Determination of gallic acid in Melastoma dodecandrum by RP-HPLC

目的建立地稔药材中没食子酸的含量测定方法,为其质量标准的建立提供科学依据。方法采用RPHPLC直接测定,色谱柱DiamonsilC18柱,流动相为四氢呋喃甲醇02%磷酸(05∶05∶99),流速1mL·min-1,检测波长274nm。结果测定了10批不同地区市售的地稔药材,没食子酸含量在0020%～0081%。结论本方法简便、快速、准确,可作为地稔药材的质量检测方法。

Objective: To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb. Method: The RP-HPLC analysis was achieved by using a Polaris C_~18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5∶0.5∶99) as the mobile phase,with a flow rate of 1.0 mL·min^-1 , and detected by UV at 274 nm. Result: The content of gallic acid was from 0.020% to 0.081%, in ten groups of M.dodecandrum collected from different locations. Conclusion: The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum. [