HPLC测定玉叶解毒颗粒中蒙花苷含量

Determination of linarin in Yuye Jiedu granule by RP-HPLC

目的用HPLC对玉叶解毒颗粒中蒙花苷含量进行测定。方法采用PhenomenexLunaC18色谱柱,以乙腈005mol·L-1磷酸氢二钠溶液磷酸(30∶70∶006)为流动相,检测波长327nm。结果蒙花苷在0025～050μg线性关系良好,r=099997,平均回收率为987%(n=5),RSD27%。结论本法简便、准确、重复性好,可用于玉叶解毒颗粒中蒙花苷的含量测定。

Objective:To Determine the content of linarin in Yuye Jiedu granule. Method:A HPLC method was developed.The chromatographic conditions are as follows Luna C_~18 column and acetonitrile-0.05 mol·L^-1 phosphate buffer-phosphoric acid(30∶70∶0.06) as mobil phase,detection wavelenth at 327 nm. Result:The linear range of linarin was 0.025-0.50 μg.The average recovery was 98.7% and RSD 2.7%.Conclusion:The method is simple and accurate, with good repeatability, and can be used for delermiuation of linarin in Yuye Jiedu granule. [