丹参酮Ⅱ_A诱导白血病细胞凋亡过程中端粒酶活性的改变

Alteration of activities of telomerase in tanshinone Ⅱ_A inducing apoptosis of the leukemia cells

目的观察丹参酮ⅡA(TanⅡA)对HL60,K562细胞端粒酶活性的抑制作用和对凋亡相关基因的影响,探讨丹参酮ⅡA对造血细胞的作用机理。方法以HL60,K562为靶细胞,应用细胞培养技术,流式细胞术,透射电镜观察TanⅡA对HL60,K562细胞的作用,利用PCRTRAP方法检测TanⅡA处理前后HL60,K562细胞端粒酶活性的改变。结果经05μg·mL-1TanⅡA作用6d后,HL60,K562细胞生长明显受到抑制,生长抑制率分别为756%和563%。经丹参酮诱导后,HL60,K562细胞发生凋亡,出现亚二倍体峰;同时显著下调HL60及K562细胞的cmyc,bcl2基因表达,上调cfos基因表达。HL60,K562细胞在TanⅡA作用后,端粒酶活性受到抑制,端粒酶活性抑制率分别为308%,508%。结论TanⅡA可明显抑制HL60和K562细胞的增殖和细胞端粒酶活性,并诱导细胞凋亡。

Objective: To investigate the effect of tanshinone Ⅱ_A on HL-60 and K562 cells apoptosis,and to assay the inhibition of the telome rase activities in the leukemia cell apoptosis induced by Tanshinone. Method: Using the techniques of cell culture in vitro,flow cytometry and PCR-TRAP observed the telomerase activities and apoptosis of HL-60 and K562 cells which treated by Tan Ⅱ_A. Result: 0.5 μg·mL^-1 Tan Ⅱ_A could obviously inhibit HL-60 and k562 cell lines growth(P<0.05), down-regulate c-myc, bcl-2 gene and up-regulate c-fos and p53 gene expression as well as induce leukemia cell apoptosis,the apoptotic rates of HL-60 and K562 cells were 11.8% and 21.8% respectively. The telomerase activities significant decreased, the inhibiting rates in HL-60 and K562 cells were 30.8% and 50.8% respectively. Conclusion: Tan Ⅱ_A could significantly inhibit the proliferation and telomerase activities of HL-60 and K562 cells and induce the leukemia cell apoptosis. [