两种^(99)Tc^m标记双功能螯合剂:NHS-MAG3和HYNIC

Two bifunctional chelating agents used in ^(99)Tc^m labeling:NHS-MAG3 and HYNIC

99Tcm在标记蛋白、核酸、多肽和抗体时,常采用双功能螯合剂作为偶联剂的间接标记法,以避免直接标记所带来的损伤。研究表明,近年来常用的两种螯合剂NHS-MAG3及HYNIC合成路线清晰,偶联及标记方法成熟,标记率、放化纯及比活度较高,为核素分子显像提供了必要的基础条件和广阔的临床应用前景。

When protein, nucleic acid, polypeptide and antibody labeled with 99Tcm, bifunctional chelating agents(BFCA)are commonly used in order to avoid damages by directly labeling. We choose two BFCA:NHS-MAG3 and HYNIC as representation. The synthesis of these two BFCA is clear and the meth-ods of conjugating and labeling are mature. Through BFCA we can gain high radiolabeling yield, radiochemi-cal purity and specific activity. The usage of BFCA can provide necessary condition for molecular imaging and extensively clinical foreground.