哮喘人群中IgE低亲和力受体基因多态性检测

Detection of the Polymorphism in Gene Coding for IgE Low-affinity Receptor in Asthma Population

目的:探讨IgE低亲和力受体(FeaRⅡ,CD23)基因点多态性与哮喘易感性的关系。方法:聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)检测外显子9上的G→A碱基突变。结果:本实验未能发现基因外显子9上存在G→A突变, PAGE电泳检测不到限制性片段长度多态性(restriction fragment length polymorphism,RFLP)。结论:所研究人群中FceRⅡ基因不存在(G→A)点多态性。

Objective: To investigate the association between the point mutation G to A which is located in exon 9 of CD23 gene and incidence of asthma. Methods: The genetic polymorphism was identified by the polymerase chain reaction (PCR) followed by restriction enzyme digestion of the amplified DNA with restriction enzyme endonuelease Hpa Ⅱ . Results: G to A mutation in exon 9 of CD23 gene was not detected in this research, and no RFLP was available in the PAGE. Conclusion: RFLP in CD23 gene was not delected in our research.