慢性阻塞性肺疾病气道炎症与痰液TNF_α、IL-8水平关系研究

Investigation of the Relationship between Chronic Obstructive Pulmonary Disease and the Values of TNFαand IL-8 in the Sputum

目的:探讨吸烟、不吸烟慢性阻塞性肺疾病(COPD)患者在稳定期及发作期痰液中IL-8和TNFα水平变化以及炎症细胞的变化与其气道炎症的关系。方法:对60例COPD患者(吸烟28例,不吸烟32例)在稳定期及发作期分别测定痰液的中性粒细胞数及IL-8和TNFα浓度。结果:吸烟组在稳定期痰液中TNFα和IL-8均较不吸烟组高(P<0.05,P<0.05);吸烟组发作期痰液中TNFα、IL-8较不吸烟组水平增高(P<0.05,P<0.05);吸烟组在发作期TNFα、IL-8水平也较稳定期显著增高(P <0.05,P<0.05);不吸烟组发作期也较同组稳定期水平显著增高(P<0.01,P<0.01)。发作期TNFα、IL-8的升高与炎症细胞数值相关(r=0.67,0.75)。结论:COPD患者痰液中IL-8和TNFα水平升高,炎症细胞增多表明气道炎症程度较重,预示COPD急性发作频率较高。

Objective: To observe the changes of TNFα. IL-8 and inflammatory cells in the sputum in smoking and none smoking patients with stable and acute chronic obstructive pulmonary disease (COPD) respectively. and to investigation the relationship between the changes and the inflammation cells cells. Methods: The number of neutrophils and the values of TNFα . IL-8 in the sputum of 60 patients with stable and acute COPD were assayed respectively ( 28 smoking patients and 32 none smoking ones). Results:Both TNFα and IL-8 in the sputum of smoking patients with stable COPD were higher than that of none smokers(P<0. 05.P<0. 05). Both TNFα and IL-8 in the sputum of smokers with acute COPD were significantly higher than that of none smoking (P<0. 05.P<0. 05). Both TNFα and IL-8 in the sputum of none smoking patients with acute COPD were significantly higher than that with stable COPD (P<0. 01,P<0. 01 ). Conclusion: The increase of TNFα,IL-8 and the numbers of inflammatory cells in the sputum of COPD patients indicate that COPD was serious and acute COPD would attack more probably.