脑脊液中高浓度锌诱导的大鼠阿尔茨海默病样行为及病理学变化

Behavior and Pathology Changes Characteristic to Alzheimer's Disease Induced by High Concentration Zinc in Cerebrospinal Fluid in Rats

目的研究脑脊液锌浓度增高诱发的大鼠阿尔茨海默病(AD)样行为学、病理学特征性改变。方法注射氯化锌溶液(ZnCl2)到大鼠脑室,用Morri's水迷宫测试行为学;用流式细胞仪测定凋亡细胞;用免疫细胞化学染色法显示β淀粉样蛋白(Aβ)的表达;透射电镜下观察超微病理改变。结果锌处理大鼠有严重的行为损害;凋亡细胞增加;大脑皮层及海马区有散在黄褐色的Aβ免疫染色斑块;电镜下可见核溶解和微管紊乱等超微病理变化。结论大鼠脑脊液中锌浓度的增高会导致类似人类AD的智力和病理学特征性改变。

Objective To study the behavior and pathology changes characteristic to Alzheimer's disease(AD) in rats induced by high concentration zinc in cerebrospinal fluid. Methods Zinc chloride was injected into the rats' cerebral ventricles. Behavioral tests were performed in the Morri's water maze (MWM). The apoptotic cells were tested by flow cytometry. Immunocytochemical staining was used to show the pathology of β-amyloid plaques. Pathology changes at cellular level were examined under transmission electron microscope (TEM). Results There was obvious behavior damage in zinc treated rats, and there were more apoptosis in zinc treated rats. β-amyloid plaques were interspersedly distributed in extensive area of cerebral cortex and hippocampus. Karyolysis and derangement of microtubules was seen in zinc treated rats. Conclusion High concentration of zinc in brain tissue may result in abnormal intelligence and pathology changes characteristic to AD.