不同方法应用静注免疫球蛋白治疗小鼠病毒性心肌炎的效果比较

Effects of Intravenous Immunoglobulin in Varying Ways on Mice with Viral Myocarditis

目的研究不同方法应用静注免疫球蛋白(IVIG)对病毒性心肌炎(VM)小鼠的疗效。方法雄性Balb/c小鼠280只随机分为7组,每组40只。A组正常对照组;B组IVIG对照组;C组病毒对照组;D组IVIG早期短程治疗组;E组IVIG早期长程治疗组;F组IVIG中期短程治疗组;G组IVIG中期长程治疗组。C^G组腹腔接种柯萨奇B3病毒01mL制成VM模型。A、B组腹腔注射09%氯化钠溶液01mL作对照。根据分组不同在接种病毒后不同时期腹腔注射不同疗程的IVIG(500mg·kg1·d1,qd)治疗。B组连用10d;D、E组接种病毒后3d始,分别用5,10d;F、G组接种病毒后7d始,分别用5,10d。A、C组给予09%氯化钠溶液01mL,ip,qd,共10d。观察小鼠死亡率,并于感染7,14,21,28d各处死6只,取心脏,分别测定心肌病毒滴度、心肌细胞凋亡率和坏死率,光镜下观察心肌病理组织学改变,计算心肌病理组织学积分。结果各IVIG治疗组(D^G组)小鼠死亡率、心肌病毒滴度、心肌病理组织学积分和心肌细胞凋亡率与坏死率均明显低于病毒对照组(C组),以早期长程治疗组(E组)最低。结论IVIG能降低VM小鼠心肌病毒滴度、心肌病理组织学积分和心肌细胞凋亡率与坏死率,从而降低小鼠死亡率。对VM具有良好疗效,以早期长程应用效果最佳。

Objective To study the effects of intraven ous immunoglobulin (IVIG) in varying ways on mice with viral myocarditis. Methods Two hundred and eighty male Balb/c mice were di vided into seven equal groups randomly (n=40): normal control ( A), IVIG contro l (B), virus control(C), IVIG early-short course treatment group (D), IVIG earl y-long course treatment group (E), IVIG middle-short course treatment group(F) , and IVIG middle-long course treatment group(G). Each mouse in C to G group wa s inoculated 0.1 mL coxsackie virus B_3 intraperitoneally, while 0.1 mL 0.9% so dium chloride injection was used in group A and B. IVIG[500 mg·(kg·d)^-1 ]were given intraperitoneally on 3rd or 7rd day after infection for 5 or 10 days, respectively, in D to G group according to the different group, while the same dosage of IVIG in group B and 0.9% sodium chloride injection in group A and C for 10 days. The mortality of mice was counted. Six mice in each group were k illed on 7th, 14th, 21st, 28th day after the infection, respectively. The virus titer in myocardium and the percentage of apoptosis and necrosis of myocytes wer e detected, myocardial histopathologic scores were counted under optical microsc ope. Results In every IVIG treatment group(D to G), the mo rtality and virus titer in myocardium, myocardial histopathologic scores, percen tage of apoptosis and necrosis of myocytes were all lower than that in virus con trol group (P<0.05), while the lowest in group E. Conclusion IVIG has good effect on mice with viral myoc arditis, and has the best result when used on 3rd day after infection for 10 day s. It can reduce the virus titer in myocardium, myocardial histopathologic score s, percentage of apoptosis and necrosis of myocytes, so lower the mortality of m ice with VM.