人白细胞介素2基因在大肠杆菌中表达水平的提高及产物初步纯化

Enhanced Expression of Human Interleukin 2 in E.coli and its Initial Purification

利用双Tac启动子和改造后的人白细胞介素2（IL－2）cDNA，提高了IL－2在大肠杆菌（E．Coli）中的表达水平，并经包涵体制备、分子重新折叠及分子筛层析等技术，对表达的IL－2进行了提取、活性恢复与纯化，纯度可达95％。本工作为用基因工程方法生产人IL－2积累了经验。

Human interleukin 2 (IL-2) has been highly expressed in E. colt by inserting IL-2 cDNA with deletion in 3' non-coding region down to double Tao promoters. The ex pressed 1L-2 forming inclusion body in bacteria cells was isolated, refolded, and subject to sephadex G-75 chromatography. As a result, the IL-2 was highly purified to 95% with the biological activity recovered. The work enriched the experience of IL-2 production by incor porating methods of genetic engineering.