扩张型心肌病患者血清中抗β受体抗体的研究

Study on Antibody against β-adrenoceptor in Sera of Patients with Dilated Cardiomyopathy

应用免疫转印法研究20例扩张型心肌病(DCM）患者血清中抗心肌抗体，发11例患者抗心肌细胞膜66.47ku肽类抗体阳性，20例正常人血清均为阴性（P＜0.01）。Betaloc（倍他乐克）抑制试验发现,Betaloc可以抑制该抗体介导的心肌细胞毒性反应。本文提示抗心肌细胞膜66.47ku肽类抗体是抗心肌β受体抗体，用免疫转印法检测抗心肌β受体抗击体，对DCM的诊断具有重要价值，Betaloc对DCM患者心肌可能具有保护作用。

Antibodies against myocardium in sera of 20 patients with dilated cardiomy opathy (DCM) were studied by using immunoblot method.The antibody against the peptide of the cardiac myocyte membrane 66.47ku was found in 11 patients with DCM,no antibody was found in sera of 20 healthy donors (P＜0.01 ).Betaloc inhibiting assay revealed that Be taloc could inhibit the antibody-mediated myocardial cytotoxic reaction in Ca^(2+)-containing medium.The above findings suggest that the antibody against the peptide of the cardiac my ocyte membrane 66.47 ku is antihody,against β-adrenoceptor.The detection of the antibody by the immunoblot method is of value in diagnosis of DCM.Betaloc may have protecting ef fect on the myocardium in patients with DCM.