呼吸道合胞病毒A、B亚型变异的进一步研究

Further Study on Antigenic Variation of Respiratory Syncytial Virus Isolated in Wuhan

利用抗呼吸道合胞病毒的单克隆抗体，通过间接免疫荧光法和免疫转印法对本室分离到的武汉地区1988年～1993年的72株呼吸道合胞病毒进行了分型，可将其分为A、B两型和B_1、B_2亚型；其中,A型株占29.2％，B型株占70.9％。流行病学分析显示，本地区呼吸道合胞病毒流行株5年中有4年以B型株为主；A、B两型的分布还具有局灶化特点；两型在性别、发病年龄方面无显著性差异。

Indirect immounofluorescence and western-blot methods with seven homemade MAbs against several virus polypeptides of respiratory syncytial virus(RSV) were used to distinguish 72 strains.Two distinct virus subgroups,A and B were identified,with subgroup B being further categorized as B_1 and B_2.These strains were isolated in Wuhan during 1988 to 1993.The RSV epidemic patterns in Wuhan were different from the European and American community and were similar to Japan.Subgroup B viruses were isolated most frequently in 4 out of 5 years(1989 to 1993)Overall,and the two subgroup have cocirculat ed within the population form Wuhan from 1989 to 1993.The prevalence of two subgroups even varied in two districts of Wuhan in the same year.It suggests the RSV outbreaks were regional and temporal.No major differences in age and gender between infants and young children infected with either subgroup were seen.