蛋白质和多肽放射性碘标记方法比对

A COMPARATIVE STUDY ON THE IODINE-LABELED METHODS OF PROTEIN AND POLYPEPTIDE

对氯胺Ｔ（ｃｈ－Ｔ）法、氯甘脲（Ｉｏｄｏｇｅｎ）法和乳过氧化物酶（Ｌａｃｔｏｐｅｒｏｘｉｄａｓｅ，简称ＬＰＯ）法进行了比较研究。结果表明：ＬＰＯ具有碘化反应温和、放射性碘利用率高、反应完全、可控性强、标记产品纯度高等优点；特别是能最大限度的保持被标记物固有的生物活性和免疫活性。

There are three methods:chloramine-T,Iodogen and lactoperoxidase(LPO).￣(125)I-ACTH,￣(125)I-in-sulin and￣(125)I-HSA are prepared by these techniques.The resuIts show that lactoperoxidase is isolatedand purified from fresh milk,meanwhile,the enzyme is used a experiment of￣(125)I-labeled protein,Pep-tide hormone and monoclone antibody,etc.LPO is a very successful meththod for it's mild,completereaction, controliable,highet labelling yield,higher purity of iodine- labeled compound and so on.Itremains biological activation and stdble character more than other two techniques.