温度敏感性聚N-异丙基丙烯酰胺水凝胶的辐射合成及其性质研究

STUDY ON SYNTHESIS AND CHARACTERISTICS OF TEMPERATURE SENSITIVE HYDROGEL POLYNIPAAm AND POLYNIPAAm/NASI BY RADIATION

采用γ辐射技术合成了温度敏感性聚N-异丙基丙烯酰胺水凝胶和N-异丙基丙烯酰胺与N-丙烯酰氧基丁二酰亚胺共聚凝胶.研究了吸收剂量、剂量率、单体与交联剂浓度等因素对合成水凝胶相转变温度(LCST)等性能的影响.结果表明.在较宽的剂量(0.5—20 kGy)、剂量率(30—90Gy/min)范围内合成的水凝胶和共聚凝胶具有很好的温度敏感性、均匀性、弹性及透明性,明显优于化学法合成的产品.还成功地用所得带活性酯基的共聚凝胶对牛血清白蛋白进行了固定化研究.

The radiation polymerization of N-isopropylacrylamide (NIPAAm) and the radiation copolymeri-zation of NIPAAm and N-acryloxysuccinimide(NASI) in aqueous solution are carried out by γ-60Cosource at room temperature. The optimum dose range is (0. 5 - 20 kGy), and the dose rate is (30 -90 Gy/min). The monomer, crosslinking concentrations are chosen. The synthesized hydrogels and copolymer hydrogels have obvious temperature sensitivity, homogeneity, and suitable mechanical properties. In addition, an active ester group is introduced into the hydrogels through radiation copolymer-ization of NIPAAm and NASI which has good temperature sensitivity. A protein, BSA is immobilized through conjugation reaction onto the activated temperature sensitive hydrogel. The conjugation yield of BSA increased with increasing of ratio of NASI in copolymer of NIPAAm and NASI.