组织微阵列研究eIF4E在鼻咽癌中的表达及其临床意义

A study of the expression and clinical significance of protein eukaryotic initiation factor 4E in nasopharyngeal carcinoma with tissue microarrays

目的 研究eIF4E在鼻咽癌及其癌旁组织和鼻咽上皮组织的表达及其临床意义。方法 应用自行研制的组织微阵列 ,结合免疫组化技术 ,检测eIF4E在鼻咽癌及其癌旁上皮组织和鼻咽上皮组织的表达情况。结果 eIF4E在鼻咽上皮组织、癌旁上皮组织和鼻咽癌组织中阳性表达率分别为 6 1 .0 %(5 8/ 95 )、84 .6 %(77/ 91 )和87.3%(1 99/ 2 2 8) ,鼻咽癌和癌旁上皮组织的阳性表达率均高于鼻咽上皮组织 (P <0 .0 1 ) ,鼻咽癌和癌旁上皮组织两者阳性表达率比较无统计学意义 (P >0 .0 5 )。eIF 4E的表达与鼻咽癌的转移和临床分期无显著相关性(P >0 .0 5 ) ,而与放疗是否耐受有显著相关 (P <0 .0 5 )。结论 eIF4E在鼻咽癌的发生过程中可能起重要作用 ;eIF4E可作为预测鼻咽癌放疗是否耐受的分子指标之一。

Objective To study the expression and clinical significance of protein eukaryotic initiation factor 4E (eIF4E) in nasopharyngeal cacinoma. Methods With the self-made tissue microarrays and immunohistochemistry, the expression of eIF4E was studied in nasopharyngeal carcinoma, epithelium tissues next to the carcinoma, and nasopharyngeal epithelium tissues. Results The positive expression rates in nasopharyngeal cacinoma, epithelium tissues next to the carcinoma, and nasopharyngeal epithelium tissues were 61.0% (58/95), 84.6% (77/91), and 87.3% (199/228) respectively. The positive expression rates in nasopharyngeal carcinoma and its side epithelium tissues were higher than that in nasopharyngeal epithelium tissues (P< 0.01). The expression of eIF4E was not correlated with the clinical staging and the metastasis (P> 0.05), but it was correlated with the radiotherapy tolerance (P< 0.05). Conclusion The eIF4E may play a major role in carcinogenesis of nasopharyngeal carcinoma, and may be one of the molecule marks to predicate the radiotherapy tolerance.