应用6种人癌基因探针进行肝癌,胃癌与脑瘤转化基因...

Study on the RFLPs and Amplification and Rearrangement of the Transforming Genes in Primary Hepatic Carcinoma Gastric Carcinoma and Brain Tumour with Six Human Oncogene Probes.

本文应用c-Ha-ras-1、N-ras wigler(左段)和P^(52-c)(右段)、c-sis、v-erbB、c-myc和v-fos六种癌基因作探针,用瑟慎印迹杂交和斑点杂交两种方法研究了94例胃癌、肝癌和脑瘤DNA的限制性片段长度多态性(RFLPs),基因扩增和基因重组情况。和85例正常人组织DNA的杂交结果相比,发现了一些国内外未曾报道过的癌基因等位片段。其中有些等位片段只在癌组织DNA中富集。两个癌患者家系中病人的一级亲属的RFLPs分析表明,这些成员中罕见的癌基因等位片段明显高于正常人群。同时我们在一些肿瘤中还发现有癌基因扩增和重排现象。

By using c-Ha-ras-1, N-ras wigler (left sequence) P^(52C-)(rigrit sequence), csis, v-erbB, c-myc and v-fos 6 oncogenes as probes, restriction fragment length polymorphisms (RFLPs) of tumour tissue DNAs of 95 patients with gastric caroinoma, primary hepatic carcinoma and brain tumour, and that of 90 normal individuals were studied With the techniques of southern blot and dot blot. simultanously, gene amplification and rceombination were also detected in some tumours. Some alleles of oncogene are first erported in Chinese population. Moreover, the chracteristies freouency of some 'rare' alleles ard genotype occured in some tumour samples is significantly higher than that occured in normal individuals. The results of pedigree analysis for 2 patients showed that some 'rare'alleles also are rich. Besides, gene amplification and recombination were found in some tumours.