摘要
从土壤中分离得到一株真菌经鉴定为简青霉Penicilliumsimplicissimum ,将该菌用于木质素类化合物利用、染料脱色、天然木素降解及产酶特性等研究 ,充分证实该菌具有木质素降解能力 .简青霉降解木质素是木质素过氧化物酶 (LiP)、漆酶(Lac)和木聚糖酶共同作用的结果 ,降解主要发生在LiP与木聚糖酶活高产的初级代谢阶段 ,与白腐真菌表现出不同的作用机制 .2 5d培养可使稻草木质素绝对量损失 0 . 2 3g ,降解率达 14. 94 % ,同时纤维素、半纤维素也有较高程度的降解 .而pH值、Cu2 + 和Mn2 + 浓度对木质素的降解有较大影响 .
A strain of fungi was isolated from soil, which was identified as Penicillium simplicissimum. This strain was capable of utilizing several lignin model compounds, making aromatic dyes decoloration and degrading natural lignin. All these results proved that Penicillium simplicissimum has ligninolytic ability. Three kinds of enzymes were believed to be the most important catalyzes in the biodegrading process. They are lignin peroxidase (LiP), laccase (Lac) and hemicellulase. And they always work synergistically. After 25 days' incubation, the amount of rice straw lignin decreased 0.23g, and the degrading rate was 14.94%. Different from the degrading mechanism of the white-rot fungi, the lignin degradation by P.simplicissimum mainly happened during the primary metabolism and it was greatly influenced by the pH of media, the concentration of Cu 2+ and Mn 2+.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2005年第2期167-171,共5页
Environmental Science
基金
国家杰出青年科学基金项目 (5 0 2 2 5 92 6)
2 0 0 0年教育部高等学校优秀青年教师教学科研奖励计划资助项目(2 0 0 1AA64 40 2 0 )
国家高技术研究发展计划 (863 )项目(2 0 0 3AA64 40 10 ) .
关键词
青霉
降解
木质素
半纤维素
Penicillium sp.
degrade
lignin
hemicellulose
