pH5条件下生物制氢反应器的启动及运行特性

Start-up and Continuous Operation of Bio-Hydrogen Production Reactor at pH5

采用连续流搅拌槽式反应器 (CSTR) ,以糖蜜废水为底物 ,探讨了pH5条件下生物制氢反应器的启动和运行特性 .结果表明 ,保持反应器内 pH为 5 ,污泥接种量为 6 g/L、COD启动负荷为 7 0kg/ (m3 ·d)、水力停留时间 (HRT)为 6h等条件 ,可在30d内完成产氢发酵菌群的驯化 .此时系统氧化还原电位 (ORP)稳定在 - 4 6 0mV～ - 4 80mV之间 ,系统呈现明显的混合酸发酵特性 .其液相末端发酵产物比例分布相当 ,乙酸、乙醇、丁酸、丙酸和戊酸含量分别占发酵产物总含量的 36 % ,33% ,18% ,13% .没有占绝对优势的发酵产物 .气相中的氢气含量 30 %～ 35 % ,其最大产氢能力为 1 3m3 / (m3 ·d) .生物制氢反应器混合酸发酵稳定运行期各种产酸发酵细菌处于均势地位 ,以球菌和杆菌为主 .

A continuous stirred-tank reactor(CSTR)for bio-hydrogen production using molasses wastewater as substrate was investigated. Emphasis was placed on assessing the start-up and continuous operation characteristics when keeping pH value constant. It was found that at pH of 5, biomass of 6g/L, organic loading rate (OLR) of 7.0kg/(m3·d) and a hydraulic retention time (HRT) of 6h, an equilibrial hydrogen-producing microbial community could be established within 30 days. Following that, oxidation redox potential (ORP) were kept within the ranges -460mV～-480mV. Typical mixed acid type fermentation was exhibited in the reactor. Little difference was observed in the distribution of liquid end products. The liquid end products proportion of the total amount was 36% of acetic acid, 33% of ethanol, 18% of butyric acid, 13% of propionic acid and valeric acid, respectively. Hydrogen content in the biogas was about 30%～35%. Maximal hydrogen production rate was 1.3m3/(m3·d). The acid-producing fermentative bacteria were in the same preponderant status when the reactor showed mixed acid type fermentation. They are mostly cocci and bacilli.