低分子RNA抑制ConA与J6－1细胞膜受体结合的研究

INHIBITON OF LOW-MOLECULAR RNA IN COMBINATION OF CONCANAVALIN A WITH RECEPTOR OF J6-1 CELL MEMBRANE

提取Ｂｒｕｋｉｔｔ淋巴瘤（Ｎａｍａｌｖａ）细胞低分子ＲＮＡ与Ｊ６－１细胞悬液培养７２小时，掺入Ｆｒｒｃ标记的ＣｏｎＡ，于荧光显微镜下观察膜荧光细胞总数和帽状细胞数。结果显示实验组帽状细胞数明显减少并呈剂量依赖关系。提示低分子ＲＮＡ对白血病细胞增殖的抑制是通过作用于细胞膜而产生效应。

The Low-Molecular RNA Extracted from brukitt lymphosarcoma namalva cells was cultinated in J6-1 cell suapension for 72 hours,and then was mixed with fluoresceinated concanavalin A.After incubation for 15 minutes of the cells of capped fluoresceinated concanavalin A and fluoresce membrane were counted. The results showed that in the experiment group there were a significant reduction of capped cells and dosage relation.It seems that the inhibitory effect of low-molecular RNA upon the cell prolireration of leukemic cells was through cell membrane.