正交试验优化超高压提取人参中人参皂苷的工艺研究

Optimizing super pressure extracting technique for ginsenoside from Panax ginseng by orthogonal test

目的 研究超高压提取人参中人参皂苷的最佳工艺。方法 采用超高压技术常温提取,正交试验优化,分光光度法检测人参总皂苷的含量。结果 超高压提取人参皂苷的最佳提取工艺参数为:提取溶剂为5 0 %乙醇,固液比为1∶75 ,提取压力为5 0 0 MPa,提取时间2 m in,人参皂苷的得率高达7.76 %。结论 超高压提取工艺具有提取效率高、时间短、能耗低、杂质含量少等优点。

Objective To study the optimum procedure of extracting ginsenoside from Panax ginseng by super pressure extracting technique (SPET). Methods SPET was used to extract ginsenoside from P. ginseng at the normal temperature. The optimum extracting process was selected by the orthogonal test. The contents of ginsenoside in P. ginseng were determined by UV-spectrophotometry. Results The optimum condition of SPET was as follows: when the solid dissolved in 50% ethanol, ratio of raw P. ginseng (g) and solvent (mL) was 1∶75, the pressure was kept at 500 MPa for 2 min; the high yield of ginsenoside was up to 7.76%. Conclusion SPET has a series of advantages, such as higher efficiency, shorter extracting time, and lower exhausting energy, at the same time, the impurity is little, etc.