霍山石斛种内遗传稳定性的RAPD初探

Hereditary stability among species of Dendrobium huoshanense by RAPD

目的 研究霍山石斛荚果内遗传稳定性及荚果间的差异大小。方法 利用从20个随机引物筛选出来的3个稳定性较好的10碱基引物对来自5个不同荚果的霍山石斛种群进行RAPD检测。结果 5个荚果内遗传相似系数较大,在92.5926%-100.00%,其中H1、H9、H10、H14存在一定程度的变异,H23是一个较为稳定的群体;5个荚果间H1、H9、H10、H23的遗传距离较小,H14与其他4个荚果的遗传距离则较大。结论 建立霍山石斛稳定的无性快繁体系是切实可行的,同时说明霍山石斛种内存在一定的变异,H14与其他荚果的差异最大。

Objective To study the hereditary stability in the pod and the diversity within the pods of Dendrobium huoshanense. Methods Three optimal decamer oligonucleotide primers with better stability selected from 20 random primers were used to detect the populations of five different pods of D. huoshanense by random amplified polymorphic DNA (RAPD). Results The coefficient of genetic similarity were higher within five pods at the range of 92.592 6%-100. 00%, the variation presented in the H1, H9, H10, and H14, but H23 was a stable group. There was a smaller genetic distance within the pods H1, H9,H10, and H23, but a bigger one between the above four and H14. Conclusion The establishment of rapid cloning system of D. huoshanense is feasible, and the results indicate that the variations exist among the above pods of D. huoshanense and the most diversity appears between H14 and the other four.