火焰原子吸收光谱法测定中草药剑花中的8种微量元素

Determination of Eight Trace Elements in the Flowers of Hylocereus Undatus by FAAS

比较了干灰化法和湿法处理样品对测定结果的影响,用火焰原子吸收光谱法对剑花中Cu,Mn,Fe,Zn,Ni,Co,Cd和Pb八种微量元素进行分析测定。结果显示,用HNO3HClO4(5∶1)混酸作消化液处理样品,精密度和回收率较好;在选定的实验条件下,剑花中各元素间相互不干扰,可在一份样品液中进行8种元素的分别测定;剑花中含有较丰富的Fe,Mn和Zn,以及Cu和Ni,未检出Co和Cd,但检出了有毒金属Pb。方法的检出限均小于0086μg·mL-1,RSD≤837%(n=8),加标回收率7969%～1186%。

The effects of different sample digestives for the flowers of hylocereus undatus are compared. Eight trace elements in the flowers of hylocereus undatus were determined by flame atomic absorption spectrophotometer. The result shows that the RSD and recovery are better if the flowers of hylocereus undatus was digested with HNO3-HClO4 (5:1) mixed acid, and the flowers of hylocereus undatus contains many essential elements among which the contents of Fe, Mn and Zn are higher than those of Ni and Cu. Co and Cd were not determined, but Ph was determined. The experimental results showed that the detection limits were all smaller than 0.086 mug.mL(-1), the RSDs (n = 8) all smaller than 8.37%, and the addition standard recovery (ASR) (n = 8) was 79.69%-118.6% for all elements.