海南快生根瘤菌蛋白、多位点酶及质粒电泳

GENOTYPIC DIVERSITY OF HAINAN FAST-GROWINg RHIZOBIA ASSESSED BY ELECTROPHORESIS OF SOLUBLE PROTEINS,PLASMIDS AND POLYLOCUS ENZYMES

在本室以前的研究中，海南快生根瘤菌具有分类上的多型性。其一部分归于已知各根瘤菌种，另有一部分菌株构成独立的表观群及相应的ＤＮＡ同源群（亚群Ⅱ和Ⅳ）。本文采用不同的电泳方法，分析了海南快生根瘤菌和已知根瘤菌种的代表共５５个菌株的全细胞蛋白、酯酶、过氧化物酶及质粒组成。结果表明：同一亚群的菌株间有相似的蛋白图谱，种群之间有较明显的差异，根瘤菌中普遍存在酯酶，酶带的数量和迁移率具有菌株专一性，更适合于苗株的鉴别。过氧化物酶只在部分菌株中观察到，亚群Ⅱ与亚群Ⅳ的菌株均显示一条酶带，但二者之间的相对迁移率有明显差别。质粒电泳中，大多数海南快生菌中存在着大质粒，分子量范围是２０－４０００Ｋｂ，同一亚群中的不同菌株间具有一定相似性，这些结果为海南快生型根瘤菌的分类提供了一些辅助证据。

The fast-growing rhizobia isolated from Hainan were divided into several groups basedon numerical taxonomy and DNA-DNA hybridization in our former researches,Some of them be-longed to known Rhizobium species ,others constructed distinctive groups including subgropupⅡ(4 strains),Ⅳ(13 strains) ,and Ⅶ(2 strains).This paper reports the soluble protein patterns,large plasmid profiles and esterasesuperoxidaes polymorphisms revealed by electrophoresis。 Theresults showed that the protein patterns were very similar among strains belonged to the samespecies,and varied among different species。 The agarose gel electrophoresis revealed most of fast-growing strains isolated from Hainan had 1 to 3 large plasmids,Although the profiles of superoxi-dase only apeared in part of tested strains,it confirmed the differentiation between subgroup Ⅱand Ⅳ by their different migrations。Esterases varied in both numbers and migration and showedstrain-specificities.