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根际优势菌耐药菌株的获得及其^(15)N标记 被引量:11

OBTAINING STREPTOMYCIN RESISTANCE STRAIN FROM PREDOMINANT RHIZOSPHERE BACTERIA AND ITS  ̄(15)N LABEL
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摘要 对两株具有反硝化活性的根际优势细菌进行了耐药性和 ̄(15)N标记;研究了该双标记菌株在土壤中的动态及其活性。结果表明,所用双标记方法是可行的,应用标记菌株可以追踪其在土壤中的消长。结果还表明,标记 ̄(15)N菌株的 ̄(15)N丰度与供试培养基中氮源的 ̄(15)N丰度相近;标记菌株的反硝化活性与出发菌株的相同。 Two strains of predominant rhizosphere bacteria having denitrifying activity were la-belled with streptomycin and with  ̄(15)N-(NH_4)_2SO_4;the dynamics and activities of these double la-belled strains in soils were investigated.The results obtained showed that the double labellingmethod used in this paper was feasible,and that the labelled strains can be used to trace thegrowth and decline of them in soils.The results also showed that the  ̄(15)N abun-abundance of  ̄(15)N-labelled strain was similar to those of the nitrogen resources in the medium tested,and that thedenitrifying activities of double labelled strain and its original atrain were the same.
出处 《微生物学通报》 CAS CSCD 北大核心 1994年第4期195-198,共4页 Microbiology China
基金 国家自然科学基金
关键词 根际细菌 耐药菌株 土壤 氮15标记 Rhizosphere bacteria,Streptomycin resistance strain, ̄(15)N label,Double labelledstrain,Denitrifying activity
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参考文献2

  • 1曹景勤,陈碧云,姚惠琴.三叶草根瘤菌耐药突变菌株共生固氮有效性的研究[J]土壤,1988(02).
  • 2姚惠琴,朱增炎,曹景勤,许月蓉,陈碧云.某些生物因子对苕子根瘤菌菌剂和土著根瘤菌竞争结瘤的影响[J]土壤学报,1983(01).

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