摘要
用B淋巴细胞杂交瘤技术制备了能稳定分泌抗脱氧雪腐镰刀菌烯醇(DON)单克隆抗体的杂交瘤细胞株,命名为3D7.3D7的亚类为lgG_1.运用该抗体,建立了检测小麦中DON的间接竞争性酶联免疫吸附测定法.该法最低检出量为5ng/ml,敏感范围为5—1000ng/ml;平均回收率为96.6—107.3%;精密度为6.2—13.3%.运用该方法,检测了10份小麦样品.均为阳性,其含量为56.4—1002.0ppb.
A hybridoma cell line secreting monoclonal antibody againtst deoxynivalenol (DON) was established by fusion of spleen cells with myeloma. The antibody was designated 3D7 with the subtype IgG1. Meanwhile, indirect competitive enzyme-linked immunosorbent assay for determination of DON in wheat was developed with this antibody. The linear range for detection of DON was 5-1 000 ng/ml and the minimum detected concentration was 5 ng/ml. The mean recovery rates and CV% from sample spiked with 5-1 000 ng/ml of DON were 96. 6-107. 3% and 5. 1-14. 2%. respectively. Analysis of 10 samples for DON showed positive results, and the concentration ranged from 56. 4-1002. 0 ppb.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第1期65-70,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金资助课题
