摘要
应用富集培养和梯度底物浓度定向筛选技术,从长期被腈化物污染的土壤中筛选到一株产 3-氰基吡啶水合酶(3-cyanopyridine hydratase)活性较高的马红球菌(Rhodococcus e-qui)SHB-121.研究了该菌3-氰基吡啶水合酶的最适形成条件.在最适条件下,酶的比活力达5.3u/mg干细胞,比在初筛条件下的酶活力提高95倍,而在其细胞内共存的尼克酰胺(烟酰胺)水解酶活力很低.
A bacterium, Rhodococcus equi SHB-121 forming 3-cyanopyridine hydratase was screened from soil polluted by nitriles according to the biomass and the enzyme activity. Its activity was the highest among 200 nitrile-utilizing bacteria isolated. The optimum formation conditions for the 3-cyanopyridine hydratase of SHB-121 have been studied. Under the optimum conditions ,5. 3 u/mg dry cell weight of specific activity was produced , and the coexistent amidase activity was very low. The specific activity of strain SHB-121 was about 95 times higher than that in primitive screening medium.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第2期131-136,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目
关键词
微生物
马红球菌
氰基吡啶
水合酶
Microbial conversion, Rhodococcus equi, 3-cyanopyridine hydratase,Nicoti-namide
