摘要
随着科学的发展与新技术在分类学中不断地应用,放线菌分类学已从经典的形态分类转向化学分类(细胞壁化学组份,磷酸类脂,枝菌酸及甲基萘醌等).现在有些国家又开展了分子分类.本实验室自80年代始开展了放线菌化学分类,建立了上述化学指征的分析方法.自90年代起,又开展了分子分类,DNA-DNA杂交、23S rRNA寡核甘酸序列分析.近来,许多人用16S rRNA部分序列区分微生物不同的基因种.作者选用了23S rRNA部分序列区分放线菌的不同属种.现将研究结果简报如下:1 材料和方法1.1菌种菌株10,13,23,C_(43),350,41,53,4650及N分离自云南省土壤中.C_(51)及3306来自日本微生物菌种保藏中心.
According to the morphological characteristics and chemotaxonomic criteria including cell wall composition,phospholipids,mycolic acids,menaquinones, 10 strains of actinomycetes were divided into 4 genera such as Streptomyces, Micromonospora, Amycolatopsis and Saccharomonospora. The result of 5' terminal of 23S r RNA sequence analysis is correspondent to the result of chemotaxonomy. Our experiment showed that 5' terminal of 23S rRNA sequence can distingush different genera of actinomycetes. There are small differences (<1 %) among strains within genus of Streptomyces (2 strains) and Saccharomonospora (7 strains).
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第3期241-244,共4页
Acta Microbiologica Sinica
基金
国家自然科学基金委重大项目资助
