两种杆状病毒的限制性消化和p10基因的定位

RESTRICTION DIGESTION OF TWO KIND OF BACULOV-IRUSES AND THE LOCATION OF THEIR p10 GENES

以5和6种限制性内切酶分别消化昆虫杆状病毒SeNPV和LsNPV DNA,求出它们的基因组平均长133kb和164kb.为了定位这两种杆状病毒的p10基因,构建了含有AcNPVp10基因序列的两个探针载体pAcHP106和pAcEP102.从探针载体回收0.18kb和0.42kb的AcNPV p10基因序列,制备探针,与SeNPV和LsNPV的酶切片段杂交,得到清晰的杂交图谱,准确的定位了这两种病毒的p10基因.

The length of SeNPV and LsNPV genomes DNA were calculated to be 133kb and 164kb respectively, according to digestion with 5-8 restriction endonuleases. In order to map p10 gene in Baculoviruses DNA by pure probe, two probe vectors, pAcHP106 and pAcEP102,were constructed with 3'-0. 18kb and 3'-0. 18kb + 5'-0. 24kb (coding sequence and promoter) from AcNPV p10 gene, The p10 genes of both baculoviruses,SeNPV and LsNPV,are located with 0. 18kb and 0. 42kb probes produced by random primer labeling.