大肠杆菌苏氨酸操纵子的体内克隆和诱变

THE CLONING OF THREONINE OPERON IN VIVO AND MUTAGENESIS IN VITRO IN E. COLI

报道了产1.2%大肠杆菌菌株的选育、野生型苏氨酸操纵子的体内克隆、体外再克隆、再克隆的体外诱变以及苏氨酸克隆pTHR12-9-1的获得.还研究了产酸克隆对产酸受体产苏氨酸的影响.发现pTHR12-9-1对低产菌有增产效应,对高产菌则相反;并对此现象作了讨论.

Report here was the selection of the E. coli strain producing 1.2% threonine. The wild type threonine operon was cloned in vivo and subcloned in vitro. The subclone was mutagenizied in vitro and a clone pTHR12-9-l producing threonine was obtained. Also the effect of pTHR12-9-l on threonine yield of hosts producing different amount of threonine was studied. We found that pTHR12-9-1 could promote the production of threonine in lower yield hosts, but it reduced the production of threonine in higher yield hosts. The phenomenon was discussed.