摘要
报道了产1.2%大肠杆菌菌株的选育、野生型苏氨酸操纵子的体内克隆、体外再克隆、再克隆的体外诱变以及苏氨酸克隆pTHR12-9-1的获得.还研究了产酸克隆对产酸受体产苏氨酸的影响.发现pTHR12-9-1对低产菌有增产效应,对高产菌则相反;并对此现象作了讨论.
Report here was the selection of the E. coli strain producing 1.2% threonine. The wild type threonine operon was cloned in vivo and subcloned in vitro. The subclone was mutagenizied in vitro and a clone pTHR12-9-l producing threonine was obtained. Also the effect of pTHR12-9-l on threonine yield of hosts producing different amount of threonine was studied. We found that pTHR12-9-1 could promote the production of threonine in lower yield hosts, but it reduced the production of threonine in higher yield hosts. The phenomenon was discussed.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第4期266-270,共5页
Acta Microbiologica Sinica
基金
安徽省省科委资助项目
关键词
苏氨酸
操纵子
体内克隆
大肠杆菌
Threonine operon, Cloning in vivo, Mutagenegis of clone in vitro.