摘要
从四川省某煤矿发热的煤矸石堆上,分离到一株极端嗜热嗜酸细菌(ES-23),其生长的温度范围为50—75℃,最适70℃;生长的pH范围为1.0—5.0,最适pH2.0.该菌株好氧,兼性自养,既能利用有机物异养生长,又能利用元素硫氧化产硫酸获得能量、固定空气中二氧化碳自养生长.革兰氏染色阴性.细胞球形或椭圆形,直径0.9—1.5μm.自养生长时细胞外有类似纤毛的附属物.细胞外被由分三层的膜构成,无细胞壁.膜脂中醚键代替酯键.DNA中G+C含量为38mol%.根据这些特征,鉴定此菌株为热原体属(Thermoplasma)的一个新种,定名为氧化硫热原体(Thermoplasma thiooxidans nov.sp.).
An extremely thermoacidophilic strain, ES-23,was isolated from a self-heating coal spoil pile, Sichuan, China. The new isolate grows between 50 and 75℃ with optimum 70℃ and pH 1. 0-5. 0 with optimum 2. 0. Strain ES-23 is aerobic and facultative autotrophic depending both heterotrophically on organic compounds and autotrophically on elemental sulfur as energy source and CO2 as carbon source. G , spherical or oval with 0. 9-1. 5μm in diameter. The autotrophic growth showed peri pilus-like structure. Cells Lack a rigid cell wall and surrounded only by a single triple layed membrane containing ether lipids. GC content of DNA is 38 mol%(Tm). It has been determined as a new species of Thermoplasma genus and named Thermoplasma thlooxidans sp. nov.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第4期255-260,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目
关键词
热原体
嗜热嗜酸菌
古细菌
新种
Thermoplasma, Thermoacidophilic, Archaebacteria