丙烯酸对十六碳二元酸发酵的影响和16L罐扩试

STUDIES ON MICROBIAL PRODUCTION OF TETRADECANE 1,14-DICARBOXYLIC ACID (DC_(16)) FROM HEXADECANE (nC_(16))

本文报道丙烯酸对发酵生产十六碳二元酸(DC_(16))的影响,加入0.1%丙烯酸,DC_(16)产量提高20—30%.在16L自动控制罐上,在最佳条件下,加入20%(v/v)正十六烷(nC_(16)),发酵5天,DC_(16)高达120g/L,nC_(16)的转化率高达79%.

The previous paper for conversion of n-hexadecane (n-C16 ) to the corresponding dicarboxylic acid(DC16) by a mutant strain UH-3-9 of Candida tropicalis in shaking flask was reported ,with 15%(v/v) of nC16,the yield of nC16 amounted to 48. 2g/L in 96 h. The present report deals with the effect of acrylic acid on nC16 fermentation by the same culture. It was found that the addition of 0. 1% of this acid increased the production of nC16 by 20-30%. In benchtype fermenter of 16L and under the optimum conditions with 20%(v/v) of nC16, pH maintained at 7. 5- 8. 0, the yield of DC16 reached 120g/L in 5 days ,the corresponding rate of conversion was 79%.