兰州市婴幼儿艰难梭菌带菌状况

Epidemiological Investigation on Clostridium difficile in Infants in Lanzhou

在兰州市随机采集的２１６名健康婴幼儿粪标本经分离并对其培养特性、菌落菌体形态特征、生化反应特性以及毒素原性等进行一系列检查，检出了３７人的粪便含有艰难梭菌（１０４─１０８／ｇ），检出总阳性率为１７．１％，新生儿、婴儿及幼儿各年龄组的检出阳性率分别为１３．５％（１９／４１）、３３．３％（１３／３９）、１３．９％（５／３６）。３７株分离菌中毒素原性阳性者仅有８株（２１．６％），均分布于婴儿与幼儿两组。新生儿１４１人中２７人的粪标本为胎粪，只有１人（３．７％）含艰难梭菌（２×１０６／ｇ），但不产毒素。所有婴幼儿中有２２人因各种原因曾用过抗菌药物，但仅有２人的粪便含艰难梭菌，而且均为非产毒株，表明艰难梭菌检出率与药物服用之间似无显著的相关性。

Fecal specimens from 216 infants aged one day to three months in Lanzhou were investigated for the presence of C. difficile and its toxin. Of these infants, 37 (17.1%) were found to be harboring C. difficile(about 104-108/g); however, none of them contain toxin in their stools.Of the.141 neonates aged less than 1 month, C. difficile was isolated from 19 (13.5%),whereas 13 (33.3%) of 19 infants aged between 1 and 12 months, were found to have C. difficile in their stools. Of the 36 infants aged between 1 and 3 years, 5 carried C. difficile in the specimens.Of the 37 isolates, only 8(21.6%) from the infants aged between 1 month and 3 years, were found to be toxigenic. Of the 27 meconiums taken from neonats, only one contained nontoxigenic C. difficile (2×106/g).Only two stool specimens from 22 infants who had used some antibiotics due to certain reasons, contained nontoxigenic C. difficile. Thus it appears that there is no Obvious relationship between the colonization of C. difficile and the medicines.