摘要
为进行ＰＣＲ实验建立了一种两步裂解血清ＨＢＶ提取其ＤＮＡ的方法。该法首先用ＳＤＳ及蛋白酶消化裂解病毒，随后再进行碱裂解，操作较酚提取法大为简化，而ＰＣＲ实验进行的两法的灵敏性比较，说明双裂解法至少不低于酚法。
A technique of the double-lysis for serum HBV DNA extraction was developed.The virus DNA was firstly disposed of by SDS and proteinase and then by alkali. The method was much simpler than the phenol extraction and its sensiticity was not lower than the latter. 10^-5 dilutions of a HBV serum sample were both determined by the two methods. the double-lysis could cooperate PCR to be applied in the clinical diagnosis.
出处
《微生物学免疫学进展》
1994年第2期22-25,共4页
Progress In Microbiology and Immunology
