合成己酸乙酯脂肪酶产生菌的筛选及产酶条件

STUDIES ON SELECTION OF LIPASE-PRODUCING MICROORGANISM AND LIPASE PRODUCTION AND ETHYL CAPROATE SYNTHESIS BY RHZOPUS SP.RH-3

从２７株脂肪酶产生菌中筛选到能由乙醇和己酸合成己酸乙酯的菌株８株。其中Ｒｈｉｚｏｐｕｓｓｐ．Ｈ－３菌株脂肪酶活力为５０－６０ｕ／ｍｌ，全细胞在有机溶剂中的酯化率可达己酸的９１％。Ｈ３产酶的最适碳源为淀粉或葡萄糖。６％黄豆饼粉加４％蛋白陈复合氮源有利于酶活力的增加。

Rhizopus sp. RH-3 found to produce large amount of lipase(50-64u/ml)。The esterification rate of its whole cells was 91% of caproic acid in organic solvent.The mediumfor high yield of lipase was composed(%)of soluable starch 1 , defatted soybean meal 6,peptone4,MgSO_4.7H_2O 0.1 , and K_2HpO_4 0.2.