马铃薯Y病毒组病毒高产量提取方法的建立

A METHOD FOR HIGH YIELD PURIFIC-ATION OF POTYVIRUSES

本文报道了高产量提取芜菁花叶病毒（ＴｕＭＶ）、莴苣花叶病毒（ＬＭＶ）、芋花叶病毒（ＤＭＶ）和大豆花叶病毒（ＳＭＶ）的提取方法。本方法通过使用高盐浓度的磷酸盐缓冲液以及在缓冲液中加入氯化镁和脲，并用ＴｒｉｔｏｎＸ－１００作为澄清剂，替代常规使用的氯仿和正丁醇，成功的提取到了大量病毒粒子，上述四种病毒提取的得率分别是ＴｕＭＶ为１７３．３ｍｇ／ｋｇ病叶，ＬＭＶ为９６ｍｇ／ｋｇ病叶，ＳＭＶ为１９９．２ｍｇ／ｋｇ病叶，ＤＭＶ为１７６．６ｍｇ／ｋｇ病叶。

This paper reported a method for high yield purification of turnip mosaic virus(TuMV),lettuce mosaic virus(LMV),dasheen mosaic virus (DMV)and soybean mosaic virus (SMV).The viruses were purified by using Triton X-100 and high molarity potassium phosphatebuffer added with magnesium chloride and urea. The yields of purified viruses were 173.3, 96,199. 2 and 176. 6 mg per kg of the infected leaves, respectively. The purified virus particles werenot aggregated. Few contaminants from host materials was observed in the purified preparationunder electron microscopy;