红假单胞菌H菌株生长细胞光照放氢条件的研究

HYDROGEN PHOTOEVOLUTION BY GROWING CULTURES OF RHODOPSEUDOMONAS sp.H

本文报道红假单胞菌属（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ）Ｈ菌株光照放氢的主要条件。结果表明，Ｈ菌株除利用苹果酸外，还可以利用葡萄糖、丁酸钠、乳酸钠等底物光照放氢，尤以葡萄糖产氢量较高。Ｈ菌株光照放氢的适宜ｐＨ为７．０，与细胞生长最适ｐＨ值一致。可以明显地抑制生长细胞放氢，在气相完全为Ｎ＿２条件下，Ｈ菌株仍能以较高速率光照放氢。Ｈ菌株光照放氢的适宜温度为３０℃。空气能抑制光照放氢，加入一定浓度的Ｎａ＿２Ｓ可以缓解这种抑制作用。

The Paper describes the experimental results of hydrogen photoevolution by growingcultures of Rhodopseztdomonas sp.H. The results showed strain H could photoevolve hydrogenfrom glucose,butyrate,lactate etc.,besides malate, with thehighcst hydrogen evolution fromglucose. The optimal pH for photoevolution of hydro9en is about 7.0. The optinial temperaturefor hydrogen eveolution is about 30℃. could depress the hydrogen evolution by growingcultures of strain H. With molecular nitrogen at the gas phase,growing culture of strain H couldevolve hydrogen. However, with air as the gas phase,hydrogen evolution by growing cultures ofstrain H was inhibited. Certain levels of Na_2S could lighten the air inhibition.