甲醛吸入对小鼠的氧化损伤作用

Oxidative damage induced by formaldehyde inhalation in mice

目的 研究甲醛吸入对小鼠肝脏、脾脏、胸腺的过氧化损伤。方法 选用健康昆明种纯系小鼠 30只 ,随机分成 5组(即阴性对照组 ,1mg/m3 ,3mg/m3 ,5mg/m3 染毒组和阳性对照组 ) ,每组 6只 ,用静式吸入染毒方式染毒甲醛 (FA)。于染毒14d后 ,测定小鼠肝脏、脾脏、胸腺中超氧化物歧化酶 (superoxidedismutase,SOD)活性和丙二醛 (maleicdialdehyde,MDA)含量。结果 随甲醛染毒剂量的增高 ,小鼠肝脏、脾脏和胸腺SOD活性呈下降趋势 ,并且有明显的剂量—效应关系 ,1mg/m3 染毒组胸腺SOD活性与阴性对照组比较差异无显著性 ,其他各组与阴性对照组比较均有显著性差异 (P <0 0 5或P <0 0 0 1) ;MDA含量各染毒组均显著高于阴性对照组 (P <0 0 5或P <0 0 0 1)。结论 气态甲醛对肝脏、脾脏、胸腺均有氧化损伤作用 。

Objective To study the oxidative damage induced by formaldehyde inhalation to liver, spleen and thymus of mice. Methods Thirty KM mice were randomly divided into 5 groups: negative control, 1 mg/m 3,3 mg/m 3,5 mg/m 3 FA inhalation and positive control groups. Mice were exposed to FA by inspiration in a static total enclosure chamber for 14 days. The activities of superoxide dismutase (SOD) and the contents of maleic dialdehyde (MDA) in liver,spleen and thymus were determined. Results A significant decline tendency of the activities of SOD was showed with increasing of doses.The results showed a significant doses-effect relationship.There was significant difference in all FA inhalation groups compared with negative control group (P<0.05 or P<0.001) except the 1 mg/m 3 group in thymus. The contents of MDA were increased significantly in all FA inhalation groups compared with negative control group (P<0.05 or P<0.001). Conclusion The gaseous formaldehyde reveales significant oxidation damage to liver,spleen and thymus of mice,and the molecular mechanism may be involved in the SOD activity repression.