反相高效液相色谱法测定肠安胶囊中黄芩苷的含量

Content Determination of Baicalin in Chang'an Capsule by RP-HPLC

目的 :建立以反相高效液相色谱法测定肠安胶囊中黄芩苷含量的方法。方法 :色谱柱为C18,流动相为甲醇 -0 07 %磷酸溶液 (44∶56),流速为1ml/min ,检测波长为280nm ,柱温为室温。结果 :黄芩苷进样量在0 1054μg～1 0540μg范围内与峰面积呈良好的线性关系 (r=0 9996) ,平均加样回收率为97 44 % (RSD=2 92 % ,n=5)。结论 :本方法简便、准确、快速 ,可用于该制剂的含量测定。

OBJECTIVE:To establish the method for determination of baicalin in Chang’an capsule by RP-HPLC. METHODS:HPLC was performed on C 18 column with methanol-0.07%phosphoric acid solution(44∶56)as mobile phase at a flow rate of lml/min and the temperature of column kept at room temperature.The UV detection wavelength was280nm.RESULTS:The linear range of baicalin was sample size0.1054μg～1.0540μg(r=0.9996),the average recovery was97.44%(RSD=2.92%,n=5).CONCLUSION:The method was simple,accurate and fast,and can be used for the determination of baicalin.