重症肌无力病人抗乙酰胆碱受体抗体的检测及其临床意义

EXAMINATION AND CLINICAL VALUES OF AchRab IN PATIENTS WITH MYASTHENIA GRAVIS

用与乙酰胆碱受体（ＡｃｈＲ）特异性结合的α－银环蛇毒素（α－ＢＧＴ）包被酶标板提纯肌肉粗提液中的ＡｃｈＲ作为抗原，按ＡＢＣ－ＥＬＩＳＡ间接法检测了５１例正常对照组，５２例临床对照组及９６例（１１０次）重症肌无力（ＭＧ）病人血清中的ＩｇＧ乙酰胆碱受体抗体（ＩｇＧ－ＡｃｈＲａｂ）。结果发现ＩｇＧ－ＡｃｈＲａｂ在ＭＧ组有３９例阳性（４４次）（４０．６３％）。临床对照组４例阳性（７．６９％）。正常对照组２例阳性，统计学处理发现ＭＧ组与其它组差异均有显著性（Ｐ＜０．０５），而临床对照组与正常对照组差异无显著性（Ｐ＞０．０５）。

Utilized α-bungarotoxin(α-BGT)capturing their corresponding specific binding proteins from a preparation of crude human muscle receptors as antigens,detected IgG antibodies to acetylcholie receptors(AchR)in sera from 96 patients(110 times)with myasthenia gravis(MG)and those from 103 controls(including 52 patients with other neurological diseases and 51 normal persons by ELISA)In MG,IgG-AchRab were positive in 39(40.63%)cases(44 times)14(7.69%)of 52 patients with other neurological diseasea had this antibody and only 2 of 51 healthy persons were positive.The level of IgG-AchRab in MG group was significantly higher than those in control group P<0.05).No significant difference was found between the two groups of controls(P<0.05).