杂交松组织培养中外植体的灭菌方法

Explant Disinfection in Tissue Culture of Pinus elliottii×P. caribaea

在实验室内对来自广西大学林学院苗圃、东门林场苗圃、广西林业局种苗基地的杂交松 (Pinus elliottii× P.caribaea)外植体进行 75 %酒精 10 s+0 .1%升汞 4～ 6 min灭菌试验。其中 ,东门林场苗圃的外植体进一步采用 75 %酒精 10 s+0 .1% Hg Cl2 6 m in结合添加入培养基中的 1.2 %大蒜液的灭菌方法进行试验。结果表明 :广西大学林学苗圃的杂交松外植体 ,采用 75 %酒精 10 s+0 .1%升汞 5～ 6 m in的灭菌方法效果较好。东门林场苗圃以及广西林业局种苗基地的杂交松外植体用 75 %酒精 +0 .1%升汞的灭菌方法效果不理想。东门林场苗圃的枝条用 75 %酒精 10 s+0 .1% Hg Cl2 6 min结合添加入培养基中的 1.2 %大蒜液的灭菌方法效果较好。

The disinfection experiments of 75% ethanol 10 seconds +0 1% HgCl 2 4~6 minutes to Pinus elliottii×P.caribaea explants from the nurseries of Forestry College(FC),Dongmen Forest Farm(DFF) and Guangxi Forestry Bureau (GFB)were performed in lab.To the explants from DFF,a further disinfection experiment was conducted in the treatment of 75% ethanol 10 seconds +0 1% HgCl 2 6 minutes with 1 2% garlic solution in the culture medium.It shows that the treatment of 75% ethanol 10 seconds +0 1% HgCl 2 5~6 minutes has better result to the explants from FC.The treatment of 75% ethanol +0 1% HgCl 2 is no good to the explants from DFF and GFB.To the explants from DFF,there is a better result in the treatment of 75% ethanol 10 seconds +0 1% HgCl 2 6 minutes with 1 2% garlic solution in the culture medium.