毛霉M263-3产蛋白酶的特性研究

On the Producing Properties of Proteinase in Mucor sp. M263-3

为了提供毛霉在工业生产中的一些基本参考数据,在不同的培养时间段对毛霉产酸、中、碱性蛋白酶的酶活性进行了测定,研究了培养基含水量和“扣瓶”处理等培养因素对产酶活力的影响,并对所产蛋白酶的耐热性进行了研究.结果表明:毛霉所产蛋白酶以碱性蛋白酶为主,在 28 ℃培养 96 h 产酶活力最高,培养基含水量以 54.7%合适,多次“扣瓶”对所产酶活有负面影响,蛋白酶在温度高于 60 ℃时容易失活.

Mucor sp.M263-3 was an important strain in food industry,it could produce high proteinase activity.In order to improve the adaptability of Mucor sp. protease production and get some basic data of Mucor sp.M263-3,its acid protease activity,neutral protease and alkaline protease was measured at different cultural time.The effect of water content,temperature and the operation of “agitation ” on protease activity was discussed.At last,the heat stability of this protease was measured.The result showed that the mainy produced protease of mucor sp.M263-3 was alkaline protease.The optimal temperature was 28 ℃,54.7 % of water content was good for protease producing.The operation of “agitation” had bad influence on the protease activity and most of protease losed its activity when temperature was above 60 ℃.