摘要
用"孢子浴"法将人工培养的新蚜虫疠霉对云南4个不同地区的烟蚜接种进行定量生物测定(25℃,光周期为L: D 12:12,RH 85％),每组包括8个剂量(0.32-51.77个分生孢子／mm2),每剂量处理烟蚜60头,逐日观察记录死亡 数至第8 d。结果表明,无论从剂量效应还是时间效应看,新蚜虫疠霉均具有强毒杀蚜特性。接种的前3 d为新蚜虫 疠霉的潜伏期,第4-6 d是对烟蚜的致死高峰期。新蚜虫疠霉引发不同地区烟蚜死亡的起始时间差别不大,经时 间-剂量-死亡率模型模拟分析表明,4地区烟蚜处理的LD50值差异不大,随时间变化趋势线在斜率和截距上也没有 明显区别。说明供试菌种对来自不同地区烟蚜种群的毒力无明显差异,目前尚不存在抗性烟蚜种群。
The entomaphthotalean fungus, Pandora neoaphidis, was bioassayed in laboratory for its virulence against 4 Yunnan geographical populations of green peach aphid, Myzus persicae. Each experimental group, consisting of 60 nymphs of the pest, was inoculated with P. neoaphidis at 8 dosages, ranging from 0. 32 to 51.77 spores/mm2 , with the ' spore shower' method. P. neoaphidis proved to be highly virulent to Myzus persicae, with a latent period of 3 days. No significant difference was detected in the virulence of fungus against various geographical populations of the pest. An analysis with the time - dose - mortality model showed no significant difference in the mortality of the pest in the four regions, suggesting that this strain of the fungus used in this study has similar virulence to the green peach aphid in the different regions studied. No resistant population of the pest has been found yet. Such results suggest that P. neoaphidis has great potential for utilization in microbial control of green peach aphid.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第1期36-39,54,共5页
Journal of Southwest Agricultural University
基金
云南省自然科学基金项目(2001 C 0017 Q)云南省科技重点攻关资助项目(2001 NG 57)
