二乙基亚硝胺诱发大鼠肝癌中间病变研究

Preneoplastic lesions of the liver in rats treated with diethylnitrosamine followed by phenobarbital

作者对人鼠肝脏的中间病变进行了研究。Ｓ．Ｄ大鼠在７０％的肝大部切除术（ＰＨ）后１８～２４小时腹腔注射１０ｍｇ／ｋｇ的二乙基亚硝胺（ＤＥＮＡ）一周后，在饮水中加入０．０５％的苯巴比妥（ＰＢ）。ＤＥＮＡ启动后１月，ｒ－ＧＴ和Ｈ·Ｅ染色可见转变灶和结节。转变灶和结节的体积随时间的延长而增加，其数量在３月未达高峰，４月末开始下降。从２月到７月，很多结节发生变性。１０月末仅在雌鼠见大的结节。这一结果支持转变灶是结节的前身，结节在一定程度上增加了恶变的危险性这一假说。实验未见在右叶和尾叶的转变灶和结节的数量差异，但结果显示雌鼠比雄鼠对化学致癌剂更敏感。

Preneoplastic lesions were investigated in rat livers.S.D.rats were initiated with of 10mg/kg of diethylnitrosamine(DENA)by I.P.injection 18￣24 h after a 70% hepatectomy.One week later,0.05%of phenobarbital (PB)was added to the drinking water.One month after initiation with DENA, foci and nodules were present in r-GT and H.E.stains. The size of foci and nodules increased with time. The number of foci and nodules was highest at the end of 3rd month and then dropped from the 4th month. From the 2nd month to 7th month ,many of the nodules underwent degeneration. At the 10th month ,persistent nodules were seen in female rats only. The result supported the hypothesis that foci were the precursor for nodules and the nodules at some extend increased risk to undergo malignant change. Comparison in the number of foci and nodules between right lobe and caudate lobe was made at the third month. No difference was found. However,the sex difference was shown. The female rats appear more sensitive to the carcinogen tested than the male rats.