用简化的原噬菌体诱导抑制试验筛检SOS反应抑制物

Detection of SOS response inhibitors with a simplified inhibitory assay of λ prophage induction

用简化的原噬菌体诱导抑制试验发现２６种抑制物，其中党参、黄精、生山楂、首乌、麦冬、浙贝母、５－氟尿嘧啶、５－氟脱氧尿苷、二氧化锗、大蒜素对４－硝基喹啉－Ｎ－氧化物、丝裂霉素Ｃ和甲基甲烷磺酸酯诱导的λｃＩ的ＳＯＳ反应有显著的抑制作用。同时，抑制作用在受试物与诱导剂及其相互间存在差异，提示受试物种类或成分及其与诱导剂间关系的复杂性，以及受试物可能以不同作用方式影响诱导过程。

SOS response inhibitors were found and 10 of them,the water extracts of Codonopsis Pilosula(Franch) Nannf,Polygonatum Sibiricum Redoute, Crataegus cuneata sieb et zucc,Polygonum multiflor Thumb, Ophiopogon japonicus Ker-Gawl, and Fritillaria verticillata willd var thunbergii Bak;and the solutions of 5-fluorouracil, 5-fluorodeoxyuridine,GeO2 and garlicin, could suppress strongly the λcI-depended sos response induced by 4-nitroqinoline-N-oxide (4NQO) , mytomycine(MMC) and methyl methansulfonate(MMS), respectively.Meanwhile, the inhibitory effects were varied with the tested samples,inductors and their correlation. It suggested that their kinds, conponent and correlation were complicated,and the tested samples probably interfered the induced SOS response by different models of action.